McGuire E A, Davis A R, Korsmeyer S J
Department of Medicine, Howard Hughes Medical Institute, Washington University, St Louis, MO.
Blood. 1991 Feb 1;77(3):599-606.
We previously identified and cloned T-cell translocation gene 1 (Ttg-1), a putative zinc finger protein, as a result of its deregulated expression in a T-cell acute lymphoblastic leukemia cell line (RPMI 8402) with a t(11;14)(p15;q11). We have now characterized its genomic organization and identified the major transcriptional start site to lie within an initiator-like motif. Ttg-1 is normally expressed in mouse brain and not in thymus. The mouse neuroblastoma cell line, N2a, also expresses Ttg-1. Antibodies raised against a TrpE-Ttg-1 fusion protein precipitate an 18-Kd nuclear protein from metabolically labeled 8402 cells. Immunofluorescence of N2a cells shows a nuclear pattern. The two potential zinc finger domains in Ttg-1 are highly homologous to similar regions in lin-11, mec-3, and lsl-1. This data suggests that Ttg-1 may be involved in gene regulation.
我们之前鉴定并克隆了T细胞易位基因1（Ttg-1），它是一种假定的锌指蛋白，其在具有t(11;14)(p15;q11)的T细胞急性淋巴细胞白血病细胞系（RPMI 8402）中表达失调。我们现在已经对其基因组结构进行了表征，并确定主要转录起始位点位于一个类似起始子的基序内。Ttg-1通常在小鼠脑中表达，而不在胸腺中表达。小鼠神经母细胞瘤细胞系N2a也表达Ttg-1。针对TrpE-Ttg-1融合蛋白产生的抗体从经代谢标记的8402细胞中沉淀出一种18-kD的核蛋白。N2a细胞的免疫荧光显示出核模式。Ttg-1中的两个潜在锌指结构域与lin-11、mec-3和isl-1中的类似区域高度同源。这些数据表明Ttg-1可能参与基因调控。
