Michaelsen T E, Garred P, Aase A
Department of Immunology, National Institute of Public Health, Oslo, Norway.
Eur J Immunol. 1991 Jan;21(1):11-6. doi: 10.1002/eji.1830210103.
The relative complement-mediated lytic capability of the IgG subclass isotypes was studied using a matched set of mouse-human chimeric anti-(4-hydroxy-3-nitrophenyl)acetyl (NP) antibodies. The subclass pattern was shown to be highly dependent on variations in antigen concentration and to lesser extent on variation in epitope patchiness, antibody binding affinity and complement concentration. In general, the IgG3 subclass was most effective in inducing cytolysis at the different conditions used and only at high antigen concentration did the IgG1 subclass mediated more efficient cytolysis than IgG3. The IgG2 isotype required a relative high antigen concentration to be cytolytic while the IgG4 isotype was not cytolytic at any of the conditions tested. These individual characters of each of the IgG subclasses makes it conceivable that a subtle system of immunoregulation exists among the subclasses.
使用一组匹配的小鼠 - 人嵌合抗（4 - 羟基 - 3 - 硝基苯基）乙酰（NP）抗体，研究了IgG亚类同种型的相对补体介导的溶解能力。结果表明，亚类模式高度依赖于抗原浓度的变化，在较小程度上依赖于表位斑块性、抗体结合亲和力和补体浓度的变化。一般来说，在所用的不同条件下，IgG3亚类在诱导细胞溶解方面最有效，只有在高抗原浓度下，IgG1亚类介导的细胞溶解才比IgG3更有效。IgG2同种型需要相对较高的抗原浓度才能具有细胞溶解作用，而IgG4同种型在任何测试条件下都没有细胞溶解作用。IgG亚类的这些个体特征使得可以想象在亚类之间存在一个微妙的免疫调节系统。
