Liu Y S, Wu J Y
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
J Appl Microbiol. 2006 Nov;101(5):1033-8. doi: 10.1111/j.1365-2672.2006.03009.x.
To identify beneficial oxygen vectors for Phaffia rhodozyma in liquid cultures, and to evaluate their use to improve the oxygen transfer and carotenoid production in the yeast cultures.
Several liquid hydrocarbons were tested as oxygen vectors for improving the yeast growth and carotenoid production in shake-flask cultures of P. rhodozyma. While all nontoxic organic liquids (Log P: > or =5.6) showed a positive effect, n-hexadecane was proved to be the most beneficial for the yeast growth and carotenoid production. The addition of 9% (v/v) n-hexadecane to the liquid medium at the time of inoculation was found to be optimal, increasing the carotenoid yield by 58% (14.5 mg l(-1) vs 9.2 g l(-1) in the control) and the oxygen transfer rate (OTR) by 90%.
The addition of n-hexadecane to shake-flask cultures of P. rhodozyma significantly improved the oxygen transfer in culture, thus increasing the carotenoid production.
Use of organic oxygen vectors such as n-hexadecane may be a simple and useful means for enhancing oxygen transfer and carotenoid production in liquid fermentation of P. rhodozyma.
确定在液体培养中对红法夫酵母有益的氧载体，并评估其用于改善酵母培养中氧传递和类胡萝卜素产量的效果。
测试了几种液态烃作为氧载体，以改善红法夫酵母摇瓶培养中的酵母生长和类胡萝卜素产量。虽然所有无毒有机液体（Log P：≥5.6）均显示出积极作用，但正十六烷被证明对酵母生长和类胡萝卜素产量最为有益。发现在接种时向液体培养基中添加9%（v/v）的正十六烷是最佳的，类胡萝卜素产量提高了58%（14.5 mg l⁻¹，而对照为9.2 g l⁻¹），氧传递速率（OTR）提高了90%。
向红法夫酵母摇瓶培养物中添加正十六烷可显著改善培养中的氧传递，从而提高类胡萝卜素产量。
使用正十六烷等有机氧载体可能是一种简单且有用的方法，可增强红法夫酵母液体发酵中的氧传递和类胡萝卜素产量。
