Morgan S J, Williams J H, Walls A F, Church M K, Holgate S T, McGill J I
Southampton Eye Hospital, England.
J Allergy Clin Immunol. 1991 Jan;87(1 Pt 1):111-6. doi: 10.1016/0091-6749(91)90221-9.
In this study we report the numbers and metachromatic dye-staining characteristics of mast cells (MCs) in the conjunctiva of normal subjects and patients with seasonal allergic conjunctivitis caused by pollenosis. In addition, we have used a monoclonal antibody to the MC-specific enzyme, tryptase, to enumerate tryptase-positive cells immunohistochemically. Tarsal conjunctival MCs were found to be present in increased numbers in the allergic compared to the nonallergic subjects. Most of the MCs exhibited staining that was formaldehyde resistant, compatible with their identification as connective tissue MCs or basophils. The high positive staining for tryptase confirmed that the metachromatic cells stained with toluidine blue were MCs and not basophils, both in normal and allergic subjects.
在本研究中，我们报告了正常受试者以及花粉症引起的季节性过敏性结膜炎患者结膜中肥大细胞（MCs）的数量和异染性染料染色特征。此外，我们使用了针对MC特异性酶类胰蛋白酶的单克隆抗体，通过免疫组织化学方法对胰蛋白酶阳性细胞进行计数。结果发现，与非过敏性受试者相比，过敏性受试者睑结膜中的MCs数量增加。大多数MCs呈现出对甲醛具有抗性的染色，这与其作为结缔组织MCs或嗜碱性粒细胞的特征相符。无论是正常受试者还是过敏性受试者，用甲苯胺蓝染色呈异染性的细胞对胰蛋白酶呈高阳性染色，这证实了这些细胞是MCs而非嗜碱性粒细胞。
