Clark J M, Rudd E
Department of Orthopaedics, University of Washington, Seattle 98195.
J Orthop Res. 1991 Mar;9(2):275-83. doi: 10.1002/jor.1100090216.
To study the distribution of cells in the surface layer of articular cartilage, rabbit hip and knee specimens were stained with silver and studied by scanning electron microscopy (SEM). The cartilage was treated en bloc using the Gomori methenamine silver technique, which stains the nuclei of exposed cells with reduced silver. The intact surface was then studied with a binocular microscope and SEM in the backscatter mode Only those cells within 30 microns of the surface stained, permitting that population to be imaged selectively. Depressions in the surface were related to groups of cells in clusters or rows bounded by collagen fibers. This study demonstrates the effectiveness of backscatter imaging in the study of chondrocytes. The relationship between surface contours and underlying cells is more complex than previously described.
为研究关节软骨表层细胞的分布情况，对兔髋关节和膝关节标本进行银染色，并通过扫描电子显微镜（SEM）进行观察。采用Gomori亚甲胺银技术对软骨进行整体处理，该技术用还原银对暴露细胞的细胞核进行染色。然后用双目显微镜和背散射模式的扫描电子显微镜对完整表面进行观察。只有距离表面30微米以内的细胞被染色，从而能够对这部分细胞群体进行选择性成像。表面的凹陷与由胶原纤维界定的成群或成排细胞有关。本研究证明了背散射成像在软骨细胞研究中的有效性。表面轮廓与下层细胞之间的关系比之前描述的更为复杂。
