Gündel J, Steinhäuser C, Matthies H
Institute for Neurobiology and Brain Research, Academy of Sciences GDR, Magdeburg.
Neurosci Lett. 1990 Nov 13;119(2):249-52. doi: 10.1016/0304-3940(90)90845-z.
Using whole cell recordings in combination with the concentration clamp technique it was shown that even enzymatically isolated hippocampal CA1 and CA3 neurons exhibit N-methyl-D-aspartate (NMDA) and L-aspartate currents. These currents were completely blocked by 0.5 mM Mg2+ or partially blocked (72.7 +/- 2.4%) by DL-2-amino-5-phosphonovalerate (50 microM) whereas 10 microM glycine (Gly) potentiated the NMDA responses (3.1 +/- 1.6-fold). A half-maximum dose of 55 microM was calculated from the sigmoid NMDA dose-response curve in the presence of 10 microM Gly. The amplitudes of these currents did not depend on the type of the proteolytic enzyme used for cell isolation.
运用全细胞记录技术结合浓度钳制技术表明，即使是经酶解分离的海马CA1和CA3神经元也表现出N-甲基-D-天冬氨酸（NMDA）电流和L-天冬氨酸电流。这些电流可被0.5 mM Mg2+完全阻断，或被DL-2-氨基-5-磷酸戊酸（50 microM）部分阻断（72.7±2.4%），而10 microM甘氨酸（Gly）可增强NMDA反应（3.1±1.6倍）。根据在10 microM Gly存在下的S形NMDA剂量反应曲线计算出半数最大剂量为55 microM。这些电流的幅度并不取决于用于细胞分离的蛋白水解酶的类型。
