Gerrits P O, Suurmeijer A J
Department of Anatomy and Embryology, University of Groningen, The Netherlands.
Am J Clin Pathol. 1991 Feb;95(2):150-6. doi: 10.1093/ajcp/95.2.150.
A standardized "low-cost" processing and embedding procedure is described for the preparation of semithin glycol methacrylate (GMA) sections of human tissue biopsy specimens for use in routine diagnostic pathology. The method is highly reliable and reproducible and is based on many years of experience in fundamental GMA research. The procedure combines the application of new, less toxic, plasticizers and the preparation of high-quality GMA mixtures. Microwave irradiation was used to improve staining qualities and to reduce staining times. An improved reticulin staining procedure is presented as well.
本文描述了一种标准化的“低成本”处理和包埋程序，用于制备人体组织活检标本的半薄甲基丙烯酸乙二醇酯（GMA）切片，以用于常规诊断病理学。该方法高度可靠且可重复，基于多年在基础GMA研究方面的经验。该程序结合了新型、低毒增塑剂的应用以及高质量GMA混合物的制备。使用微波辐射来提高染色质量并减少染色时间。还介绍了一种改进的网状纤维染色程序。
