Gray G E, Sanes J R
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110.
Neuron. 1991 Feb;6(2):211-25. doi: 10.1016/0896-6273(91)90357-6.
We used retrovirus-mediated gene transfer to study the migration of clonally related cells in the developing chicken optic tectum. Clonal cohorts initially form radial arrays in the ventricular zone (approximately E5), but eventually divide into three separate migratory streams. In the first migration, a minor population of cells migrates tangentially along axon fascicles in medio-laterally directed files (approximately E6-E7); these eventually differentiate into multipolar efferent cells. After E7, the majority of cells in each clone migrate radially along fascicles of radial glia to form the tectal plate, wherein they differentiate into neurons and astrocytes. Around E9, a set of small cells leaves the radial arrays in superficial layers to form a second tangential migration; at least some of these differentiate into astrocytes. Thus, as the tectum develops, cells derived from a single multipotential precursor migrate along three separate pathways, follow separate guidance cues, and adopt distinct phenotypes.
我们利用逆转录病毒介导的基因转移来研究发育中的鸡视顶盖中克隆相关细胞的迁移。克隆群体最初在脑室区（约E5）形成放射状排列，但最终分为三个独立的迁移流。在第一次迁移中，一小部分细胞沿着轴突束以中侧向排列的方式进行切向迁移（约E6-E7）；这些细胞最终分化为多极传出细胞。E7之后，每个克隆中的大多数细胞沿着放射状胶质细胞束进行径向迁移，形成顶盖板，在其中它们分化为神经元和星形胶质细胞。大约在E9时，一组小细胞离开表层的放射状排列，形成第二次切向迁移；其中至少一些细胞分化为星形胶质细胞。因此，随着顶盖的发育，源自单个多能前体的细胞沿着三条独立的途径迁移，遵循不同的引导线索，并呈现出不同的表型。
