一种新的缺陷噬菌体，其包含在纳豆芽孢杆菌菌株中可诱导的宿主染色体DNA的一个随机选择的8千碱基对片段。

A new defective phage containing a randomly selected 8 kilobase-pairs fragment of host chromosomal DNA inducible in a strain of Bacillus natto.

作者信息

Tsutsumi Y, Hirokawa H, Shishido K

机构信息

Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.

出版信息

FEMS Microbiol Lett. 1990 Oct;60(1-2):41-6. doi: 10.1016/0378-1097(90)90341-m.

DOI:10.1016/0378-1097(90)90341-m

PMID:1704337

Abstract

A new defective phage, designated PBND8, was induced in Bacillus natto strain IAM1207 with bleomycin and mitomycin C. PBND8 particles contained a randomly selected 8 kilobase-pairs (kbp) fragment of the host chromosomal DNA. Electron microscopy showed that PBND8 has a small head with a complex tail structure like PBSX, a defective phage of Bacillus subtilis 168. The PBND8 head, however, is clearly smaller than that of PBSX which contains 13-kbp fragments of the host chromosomal DNA. SDS-polyacrylamide gel electrophoretic analysis revealed that the structural proteins of PBND8 are distinct from those of PBSX and PBSY (PBSZ) of B. subtilis W23. PBND8 exhibited a bacteriocin-like killing activity to the other Bacillus cells.

摘要

用博来霉素和丝裂霉素C在纳豆芽孢杆菌IAM1207菌株中诱导出一种新的缺陷噬菌体，命名为PBND8。PBND8颗粒含有宿主染色体DNA随机选择的8千碱基对（kbp）片段。电子显微镜显示，PBND8有一个小头和一个复杂的尾部结构，类似于枯草芽孢杆菌168的缺陷噬菌体PBSX。然而，PBND8的头部明显小于含有宿主染色体DNA 13-kbp片段的PBSX。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析表明，PBND8的结构蛋白与枯草芽孢杆菌W23的PBSX和PBSY（PBSZ）不同。PBND8对其他芽孢杆菌细胞表现出类细菌素的杀伤活性。