Liubomirskaia N V, Arkhipova I R, Il'in Iu V
Genetika. 1990 Oct;26(10):1720-8.
The Drosophila melanogaster cultured cells were subjected to stable transformation by cotransfection with two plasmids, one of which conferred G418 resistance and the second contained the Drosophila retrotransposon MDG4 (gypsy) under control of different promoter fragments of the heat shock protein gene hsp70. Transcription of these constructs as well as of the endogenous gypsy was examined in the conditions of heat shock. Active degradation of preexisting MDG4 transcripts is observed after heat shock. Transcription of MDG4 is restored during recovery but its termination and/or 3' end processing becomes aberrant.
将两个质粒共转染,使果蝇培养细胞进行稳定转化。其中一个质粒赋予细胞对G418的抗性,另一个质粒含有果蝇逆转座子MDG4(gypsy),其受热休克蛋白基因hsp70不同启动子片段的控制。在热休克条件下检测这些构建体以及内源性gypsy的转录情况。热休克后观察到先前存在的MDG4转录本发生了活性降解。MDG4的转录在恢复过程中得以恢复,但其终止和/或3'端加工变得异常。