Lo Ken C, Rossi Michael R, Burkhardt Tania, Pomeroy Scott L, Cowell John K
Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
Genes Chromosomes Cancer. 2007 Jan;46(1):53-66. doi: 10.1002/gcc.20388.
Combined analysis of gene expression array data and array-based comparative genomic hybridization data have been used in a series of 26 pediatric brain tumors to define up- and downregulated genes that coincide with losses, gains, and amplifications involving specific chromosome regions. Frequent losses were defined in chromosome arms 3q, 6q, 8p, 10q, 16q, 17p, and gains were identified in chromosome 7, and chromosome arms 9p and 17q. Amplification of a 2p region was seen in only one tumor, which corresponded to increased expression of the MYCN and DDX1 genes. To facilitate the analysis of the two data sets, we have developed a custom overlay tool that defines genes that are underexpressed in regions of deletions and overexpressed in regions of gain, across the genome and specifically within regions showing recurrent involvement in medulloblastomas.
基因表达阵列数据与基于阵列的比较基因组杂交数据的联合分析已应用于26例儿童脑肿瘤，以确定与特定染色体区域的缺失、增加和扩增相一致的上调和下调基因。在染色体臂3q、6q、8p、10q、16q、17p中发现频繁缺失，在染色体7以及染色体臂9p和17q中发现增加。仅在一个肿瘤中观察到2p区域的扩增，这与MYCN和DDX1基因的表达增加相对应。为便于对这两个数据集进行分析，我们开发了一种定制的叠加工具，该工具可定义在全基因组以及特别是在髓母细胞瘤中反复受累区域内，在缺失区域表达下调而在增加区域表达上调的基因。
