中国北方汉族人群中G蛋白偶联受体激酶4基因变异与原发性高血压的关联研究

Association study of G protein-coupled receptor kinase 4 gene variants with essential hypertension in northern Han Chinese.

作者信息

Wang Yaping, Li Biao, Zhao Weiyan, Liu Pei, Zhao Qi, Chen Shufeng, Li Hongfan, Gu Dongfeng

机构信息

Division of Population Genetics and Prevention, Cardiovascular Institute, Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 167 Beilishi Road, Beijing 100037, China.

出版信息

Ann Hum Genet. 2006 Nov;70(Pt 6):778-83. doi: 10.1111/j.1469-1809.2006.00278.x.

DOI:10.1111/j.1469-1809.2006.00278.x

PMID:17044852

Abstract

To investigate the association between polymorphisms in the G protein-coupled receptor kinase 4 gene (GRK4) (R65L, A142V and A486V) and essential hypertension in northern Han Chinese, we conducted a case-control study consisting of 503 individuals with essential hypertension (HT) and 490 age-, gender-, and area-matched normotensive (NT) controls. The three GRK4 variants were genotyped by PCR-RFLP analysis. Both haplotype and single locus analysis were used to process the genotyping data. The A486 allele showed a significant association with HT (P < 0.001). A total of 6 haplotypes were observed in the entire population, with the haplotypes L-V-A and R-A-A being found to be significantly related to hypertension (P= 0.001).

摘要

为了研究G蛋白偶联受体激酶4基因（GRK4）多态性（R65L、A142V和A486V）与中国北方汉族人群原发性高血压之间的关联，我们进行了一项病例对照研究，该研究包括503例原发性高血压（HT）患者和490例年龄、性别及地区匹配的血压正常（NT）对照者。通过聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）对GRK4基因的三个变体进行基因分型。单倍型和单基因座分析均用于处理基因分型数据。A486等位基因与原发性高血压显著相关（P < 0.001）。在整个人群中共观察到6种单倍型，发现单倍型L-V-A和R-A-A与高血压显著相关（P = 0.001）。