Shi Xiao-yan, Hu Guo-qing, Yuan Xiang-lin, Li Hong-yu, Liu You-qing, Ma Ding
Molecular Cancer Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhonghua Zhong Liu Za Zhi. 2006 May;28(5):364-7.
To detect the expression of VEGF-C in nasopharyngeal carcinoma (NPC) and explore its relationship with proliferation and metastasis of NPC.
Biopsy specimens of 62 NPC patients were divided into 2 equal portions, one for immunohistochemical staining with VEGF-C polyclonal antibody by streptavidin peroxidase method, another for flow cytometry with Ki67 antibody to analyze the proliferation of tumors. The patients were followed up periodically, and then their 3-year survival and the cause of death were statistically analyzed.
Of the 62 patients, the percentage of VEGF-C positive cells in the tumors ranged from 0 - 82%, 17 (27.4%) were negative, 13 (21.0%) weak positive, 18 (29.0%) moderate positive and 14 (22.6%) strong positive. Ki67 positive tumor cells ranged from 0 - 52%, 40 cases (64.5%) showed low expression which include 15 cases of negative, 22 (35.5%) showed high expression. There was a direct relationship between the expression of VEGF-C and Ki67 (r = 0.323, P < 0.05). The 3-year survival rate of 62 patients was 66.1%. The expression of VEGF-C in the patients with positive lymph node was much higher than that with negative lymph node (P < 0.01). Among the 8 patients with distant metastasis, their VEGF-C expression was strong positive while among the 21 disease free survival patients, their VEGF-C expression was (-) or (+) account for 80.9%. An inverse correlation was found between the VEGF-C expression and prognosis of NPC patients, but the difference has no statistical significance (r = -0.219, P > 0.05).
High expression of VEGF-C is related with proliferation and metastasis of NPC cells. It is not an independent prognostic factor, but a predictive marker of disease-free survival of NPC patients.
检测血管内皮生长因子-C（VEGF-C）在鼻咽癌（NPC）中的表达，探讨其与NPC增殖和转移的关系。
将62例NPC患者的活检标本分成两份，一份采用链霉亲和素过氧化物酶法用VEGF-C多克隆抗体进行免疫组化染色，另一份用Ki67抗体进行流式细胞术分析肿瘤增殖情况。对患者进行定期随访，然后对其3年生存率及死亡原因进行统计学分析。
62例患者中，肿瘤组织中VEGF-C阳性细胞百分比为0 - 82%，17例（27.4%）为阴性，13例（21.0%）为弱阳性，18例（29.0%）为中度阳性，14例（22.6%）为强阳性。Ki67阳性肿瘤细胞为0 - 52%，40例（64.5%）呈低表达，其中15例为阴性，22例（35.5%）呈高表达。VEGF-C表达与Ki67之间存在直接关系（r = 0.323，P < 0.05）。62例患者的3年生存率为66.1%。有淋巴结转移患者的VEGF-C表达明显高于无淋巴结转移患者（P < 0.01）。8例有远处转移的患者中，VEGF-C表达为强阳性，而在21例无病生存患者中，其VEGF-C表达为（-）或（+）的占80.9%。VEGF-C表达与NPC患者预后呈负相关，但差异无统计学意义（r = -0.219，P > 0.05）。
VEGF-C高表达与NPC细胞的增殖和转移有关。它不是独立的预后因素，而是NPC患者无病生存的预测指标。
