Taléns-Visconti R, Bonora A, Jover R, Mirabet V, Carbonell F, Castell J V, Gómez-Lechón M J
Centro de Investigación, Hospital La Fe, Valencia, Spain.
Toxicol In Vitro. 2007 Mar;21(2):324-9. doi: 10.1016/j.tiv.2006.08.009. Epub 2006 Sep 5.
Adipose tissue represents an accessible source of mesenchymal stem cells (ADSCs), with similar characteristics to bone marrow-derived stem cells. The aim of this work was to investigate the transdifferentiation of ADSCs into hepatic lineage cells in vitro. ADSCs were obtained from human adipose tissue from lipectomy. Cells were grown in medium containing 15% AB human serum. Cultures were serum deprived for two days and exposed to a two-step protocol with two different media using growth factors and cytokines. Hepatic differentiation was assessed by RT-PCR of liver-marker genes. ADSCs exhibited a fibroblastic morphology that changed to a cuboidal shape when cells differentiated. Expression of liver genes increased when using one of the two studied media consisting of DMEM supplemented with HGF, bFGF and nicotinamide for 14 days. The results indicate that, under certain specific inducing conditions, ADSCs can be induced to differentiate into hepatic lineage in vitro. Adipose tissue may be an ideal source of high amounts of autologous stem cells.
脂肪组织是间充质干细胞（ADSCs）的一个可获取来源，其特性与骨髓来源的干细胞相似。本研究的目的是在体外研究ADSCs向肝系细胞的转分化。ADSCs取自抽脂术后的人体脂肪组织。细胞在含有15% AB人血清的培养基中培养。培养物血清饥饿处理两天，然后使用生长因子和细胞因子，在两种不同的培养基中进行两步培养方案。通过肝标志物基因的RT-PCR评估肝分化情况。ADSCs呈现成纤维细胞形态，分化时细胞形态变为立方形。当使用两种研究培养基之一（由补充有HGF、bFGF和烟酰胺的DMEM组成）培养14天时，肝脏基因的表达增加。结果表明，在某些特定的诱导条件下，ADSCs可在体外被诱导分化为肝系细胞。脂肪组织可能是大量自体干细胞的理想来源。
