批量形式及药物制剂中抗糖尿病药物格列吡嗪的溶出伏安法定量分析。
Stripping voltammetric quantification of the anti-diabetic drug glipizide in bulk form and pharmaceutical formulation.
作者信息
Ghoneim E M, El-Attar M A, Hammam E, Khashaba P Y
机构信息
Chemistry Department, Faculty of Science, Tanta University, Tanta, Egypt.
出版信息
J Pharm Biomed Anal. 2007 Mar 12;43(4):1465-9. doi: 10.1016/j.jpba.2006.09.025. Epub 2006 Oct 12.
The electrochemical behavior of glipizide at the hanging mercury drop electrode (HMDE) was studied in B-R universal buffers of pH 1.7-11. The voltammograms exhibited a well-defined 4-electron irreversible cathodic peak which attributed to reduction of the two C=N of the pyrazine ring of glipizide molecule. Glipizide was found to has an interfacial adsorptive character onto the mercury electrode surface. A monolayer surface coverage of 1.02x10(-10)mol cm(-2) was estimated and hence each adsorbed glipizide molecule occupied an area of 1.63 nm(2) onto the mercury electrode surface. A simple and precise square-wave adsorptive cathodic stripping (SWAdCS) voltammetric procedure was described for quantification of bulk glipizide with a limit of detection of 1.5x10(-10)M and a limit of quantitation of 5x10(-10)M. The proposed procedure was successfully applied for quantitation of glipizide in its pharmaceutical formulation (Minidiab tablets) without interference from excipients.
在pH值为1.7 - 11的B - R通用缓冲溶液中研究了格列吡嗪在悬汞滴电极（HMDE）上的电化学行为。伏安图显示出一个明确的4电子不可逆阴极峰，这归因于格列吡嗪分子吡嗪环上两个C=N的还原。发现格列吡嗪在汞电极表面具有界面吸附特性。估计单层表面覆盖度为1.02×10⁻¹⁰ mol·cm⁻²，因此每个吸附的格列吡嗪分子在汞电极表面占据的面积为1.63 nm²。描述了一种简单而精确的方波吸附阴极溶出伏安法（SWAdCS）用于定量测定格列吡嗪，检测限为1.5×10⁻¹⁰ M，定量限为5×10⁻¹⁰ M。所提出的方法成功应用于其药物制剂（美吡达片）中格列吡嗪的定量测定，不受辅料干扰。
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