多能成人祖细胞的分离与培养程序。
Multipotent adult progenitor cell isolation and culture procedures.
作者信息
Breyer April, Estharabadi Nima, Oki Masayuki, Ulloa Fernando, Nelson-Holte Molly, Lien Laura, Jiang Yuehua
机构信息
University of Minnesota, Stem Cell Institute, Minneapolis, MN 55455, USA.
出版信息
Exp Hematol. 2006 Nov;34(11):1596-601. doi: 10.1016/j.exphem.2006.07.013.
PMID:17046581
Abstract
We describe methods for isolation of multipotent adult progenitor cells (MAPCs) from newborn to 6-week-old mice and rats. The maintenance of these cells, including their culture, media formulas, and quality control procedures, are also explained. Additionally, ways to identify MAPCs including their phenotype and morphology are discussed.
摘要
我们描述了从新生至6周龄小鼠和大鼠中分离多能成年祖细胞（MAPC）的方法。还解释了这些细胞的维持方法，包括其培养、培养基配方和质量控制程序。此外，还讨论了鉴定MAPC的方法，包括其表型和形态。
Zotero 插件