Gruber Helen E, Ingram Jane A, Hanley Edward N
Orthopaedic Research Biology, Cannon Research Center, 3rd floor, Carolinas Medical Center, PO Box 32861, Charlotte, NC 28232, USA.
Spine (Phila Pa 1976). 2006 Oct 15;31(22):2556-61. doi: 10.1097/01.brs.0000241117.31510.e3.
Human intervertebral disc tissue from the anulus was obtained in a prospective study investigating the presence of the matricellular protein thrombospondin (TSP) in human and sand rat discs. Studies were approved by the authors' Human Subjects Institutional Review Board and Institutional Animal Care and Use Committee.
To determine whether TSP could be detected in the human or sand rat disc with immunohistochemistry, and to assess its localization.
The role of the matricellular proteins in maintenance of disc health and extracellular matrix remodeling is as yet poorly understood. SPARC and tenascin have previously been shown to be present in the human disc. TSP has a well-recognized antiangiogenic activity in vivo and in vitro.
Sixteen specimens of human disc tissue and discs from 7 sand rats were assessed for immunohistochemical localization of TSP. Three human disc cell cultures grown in three-dimensional culture were also evaluated. RESULTS.: Strong immunoreactivity was present in the outer anulus of both human and sand rat discs. Inner anulus showed lesser localization. In clusters, both immuno-positive and -negative cells were present. Similar patterns of localization were seen in the sand rat specimens. Human disc cells in three-dimensional culture produced abundant TSP.
The biologic basis for the avascular adult human disc does not appear to have been explored. Since TSP has recognized antiangiogenic effects both in vitro and in vivo, we suggest that the strong immunolocalization of TSP in the outer anulus indicates a role for TSP in the avascular status of the adult human and sand rat disc.
在一项前瞻性研究中获取了人类椎间盘纤维环组织，该研究旨在调查基质细胞蛋白血小板反应蛋白（TSP）在人类和沙鼠椎间盘中的存在情况。研究得到了作者所在机构的人体研究机构审查委员会和机构动物护理与使用委员会的批准。
通过免疫组织化学方法确定人类或沙鼠椎间盘中是否能检测到TSP，并评估其定位。
基质细胞蛋白在维持椎间盘健康和细胞外基质重塑中的作用目前仍知之甚少。此前已证明人类椎间盘中存在SPARC和腱生蛋白。TSP在体内和体外均具有公认的抗血管生成活性。
对16份人类椎间盘组织标本和7只沙鼠的椎间盘进行TSP免疫组织化学定位评估。还对在三维培养中生长的三种人类椎间盘细胞培养物进行了评估。结果：人类和沙鼠椎间盘的纤维环外层均存在强免疫反应性。纤维环内层的定位较少。细胞呈簇状分布，既有免疫阳性细胞，也有免疫阴性细胞。沙鼠标本中观察到类似的定位模式。三维培养中的人类椎间盘细胞产生大量TSP。
成人人类无血管椎间盘的生物学基础似乎尚未得到探索。由于TSP在体外和体内均具有公认的抗血管生成作用，我们认为TSP在纤维环外层的强免疫定位表明TSP在成人人类和沙鼠椎间盘的无血管状态中发挥作用。
