Gruber Helen E, Ingram Jane A, Norton H James, Hanley Edward N
Department of Orthopaedic Surgery, Carolinas Medical Center, Charlotte, NC 28232, USA.
Spine (Phila Pa 1976). 2007 Feb 1;32(3):321-7. doi: 10.1097/01.brs.0000253960.57051.de.
Human intervertebral disc anulus tissue was obtained in a prospective study of cell senescence. Localization of the senescence biomarker beta-galactosidase (senescence associated beta-galactosidase, SA-beta-gal) was used for quantitative determination of the % senescent cells. Discs were obtained from surgical specimens or control donors. Discs were also studied from the lumbar spine of the sand rat. Experimental studies were approved by the authors' Human Subjects Institutional Review Board and animal use committee.
To determine the incidence of cell senescence in human discs with Thompson Grades I through V using immunocytochemistry to quantify the percentage of cells positive for the senescence biomarker SA-beta-gal.
Cell senescence has been recognized as a potential factor playing a role age-related disc degeneration. Senescent cells are viable but have lost the ability to divide. Senescence cells, however, are metabolically active.
Fifty-seven discs specimens from 54 subjects were examined with immunocytochemistry for anti-SA-beta-gal immunocytochemical localization to identify senescent cells. The fraction of positive cells was determined with quantitative histomorphometry.
Quantitative histomorphometry of human discs show an overall incidence of SA-beta-gal-positive cells of 29.9% (+/-24.8, SD), with a range from 0 to 92.01%. Analysis by ANOVA of the % senescent cells grouped by Thompson grade showed significant increases in senescence with increasing disc degeneration (P < 0.0001). Further analysis with Tukey's test showed significant differences between the % senescent cells in Grades I/II versus IV, and versus V. SA-beta-gal-positive cells were also present in discs of the aging sand rat spine.
Quantitative analysis of immunohistochemical localization of SA-beta-gal identified a sizeable population of senescent cells in the aging/degenerating disc. It is important to discover more about the senescent disc cell population because these cells persist and accumulate over time within the disc. Since senescent cells cannot divide, senescence may reduce the disc's ability to generate new cells to replace existing ones lost to necrosis or apoptosis.
在一项关于细胞衰老的前瞻性研究中获取了人类椎间盘纤维环组织。利用衰老生物标志物β - 半乳糖苷酶（衰老相关β - 半乳糖苷酶，SA - β - gal）的定位来定量测定衰老细胞的百分比。椎间盘取自手术标本或对照供体。还对沙鼠腰椎的椎间盘进行了研究。实验研究经作者所在机构的人体研究伦理审查委员会和动物使用委员会批准。
使用免疫细胞化学方法量化衰老生物标志物SA - β - gal阳性细胞的百分比，以确定汤普森分级为I至V级的人类椎间盘中细胞衰老的发生率。
细胞衰老已被认为是与年龄相关的椎间盘退变的一个潜在因素。衰老细胞是有活力的，但已失去分裂能力。然而，衰老细胞代谢活跃。
对来自54名受试者的57个椎间盘标本进行免疫细胞化学检测，以检测抗SA - β - gal免疫细胞化学定位，从而识别衰老细胞。通过定量组织形态计量学确定阳性细胞的比例。
人类椎间盘的定量组织形态计量学显示，SA - β - gal阳性细胞的总体发生率为29.9%（±24.8，标准差），范围为0至92.01%。按汤普森分级对衰老细胞百分比进行方差分析显示，随着椎间盘退变加重，衰老显著增加（P < 0.0001）。进一步用Tukey检验分析显示，I/II级与IV级以及与V级之间衰老细胞百分比存在显著差异。衰老沙鼠脊柱的椎间盘中也存在SA - β - gal阳性细胞。
对SA - β - gal免疫组织化学定位的定量分析确定了衰老/退变椎间盘中存在相当数量的衰老细胞。进一步了解衰老的椎间盘细胞群体很重要，因为这些细胞在椎间盘中会随着时间持续存在并积累。由于衰老细胞不能分裂，衰老可能会降低椎间盘产生新细胞以替代因坏死或凋亡而丢失的现有细胞的能力。
