Stone S F, Zeisler R, Gordon G E
Center for Analytical Chemistry, National Institute of Standards and Technology, Gaithersburg, MD 20899.
Biol Trace Elem Res. 1990 Jul-Dec;26-27:85-91. doi: 10.1007/BF02992661.
A combination of two methods, polyacrylamide gel electrophoresis (PAGE) and neutron activation analysis (NAA), has been applied to solutions containing phosphoproteins for the purpose of protein quantification. The proteins were separated by molecular weight using PAGE, and then the whole gel was activated by neutron bombardment. Densitometric measurements of the developed bands from 32P, taken from autoradiographs of the activated gels, resulted in quantification of the phosphorus, and then the related protein. This PAGE/NAA method was applied to several phosphoprotein-containing materials, including commercial milk products and reference materials, i.e., IAEA A-11, milk powder, and SRM 1845, Cholesterol in Egg Powder.
为了进行蛋白质定量，将聚丙烯酰胺凝胶电泳（PAGE）和中子活化分析（NAA）这两种方法结合应用于含磷蛋白的溶液。使用PAGE按分子量分离蛋白质，然后通过中子轰击使整个凝胶活化。对活化凝胶的放射自显影片上32P显影条带进行光密度测量，从而对磷进行定量，进而对相关蛋白质进行定量。这种PAGE/NAA方法应用于几种含磷蛋白的材料，包括市售乳制品和参考物质，即国际原子能机构A - 11、奶粉以及SRM 1845（蛋粉中的胆固醇）。
