van Oss C J, Arnold K, Coakley W T
Department of Microbiology, Suny, Buffalo 14214.
Cell Biophys. 1990 Aug;17(1):1-10. doi: 10.1007/BF02989801.
At dextran (Mw approximately 500,000) concentrations from 2 to approximately 10%, suspensions of normal human erythrocytes flocculate in small convex agglutinates. At dextran concentrations greater than 10%, the erythrocytes resegregate in a stable monodisperse suspension. At all these dextran concentrations, the erythrocytes are coated with considerable amounts of dextran. It can be argued that at dextran concentrations from 2 to 10%, as well as at dextran concentrations greater than 10%, there is a thin layer, which is depleted of dextran, between the dextran layer adsorbed onto the erythrocytes and the bulk dextran solution. It can also be shown that there is a repulsive interaction between the two layers of dextran: one adsorbed and one free. When the adsorbed dextran layer is the most concentrated, stability must ensue, and when the dextran in free solution is the most concentrated, flocculation should occur. Below 7% dextran, the concentration of free dextran is higher than the adsorbed concentration; above 10% dextran that situation is reversed. These data correlate well with the depletion flocculation predicted for the lower concentration and the depletion stabilization predicted for the higher dextran concentration.
在葡聚糖（分子量约500,000）浓度为2%至约10%时，正常人红细胞悬液会絮凝成小的凸起凝集物。当葡聚糖浓度大于10%时，红细胞会重新分离形成稳定的单分散悬液。在所有这些葡聚糖浓度下，红细胞都被大量的葡聚糖所覆盖。可以认为，在葡聚糖浓度为2%至10%时，以及在葡聚糖浓度大于10%时，吸附在红细胞上的葡聚糖层与大量葡聚糖溶液之间存在一层葡聚糖耗尽的薄层。还可以证明，两层葡聚糖之间存在排斥相互作用：一层被吸附，一层是游离的。当吸附的葡聚糖层浓度最高时，必然会产生稳定性，而当游离溶液中的葡聚糖浓度最高时，就会发生絮凝。在葡聚糖浓度低于7%时，游离葡聚糖的浓度高于吸附浓度；在葡聚糖浓度高于10%时，情况则相反。这些数据与较低浓度下预测的耗尽絮凝以及较高葡聚糖浓度下预测的耗尽稳定化密切相关。
