Yang Shi-hai, Liu Xiao-feng, Shen Xin, Zheng Jun-hua
College of Chinese Medicinal Material, Jilin Agricultural University, Changchun 130118, China.
Zhongguo Zhong Yao Za Zhi. 2006 Jun;31(11):875-8.
To study the induction of hairy roots of Glycyrrhiza uralensis and effect of some physical as well as chemical factors on the growth of hairy roots.
Hypocotyls and cotyledons of G. uralensis were infected with Agrobacterium rhizogenes LBA9402 and A. rhizogenes R1601, respectively.
Hairy roots were induced by two strains of A. rhizogenes, and LBA9402 showed astronger infective ability. The frequency of transformation of hypocotyls was higher than that of cotyledons. Among the different media tested, WP medium yielded the fastest growth rate of hairy roots. Light inhibited the growth of hairy roots. The total amout of 5 flavonoids in hairy roots was 1.5 times as much as that in the callus, among them licochalcone content was 15.5 times as that of callus.
The in vitro culture system of the hairy roots established laid a foundation for mass production of flavonoids by hairy root culture.
研究乌拉尔甘草毛状根的诱导以及一些物理和化学因素对毛状根生长的影响。
分别用发根农杆菌LBA9402和发根农杆菌R1601感染乌拉尔甘草的下胚轴和子叶。
两种发根农杆菌菌株均能诱导出毛状根，且LBA9402的感染能力更强。下胚轴的转化频率高于子叶。在所测试的不同培养基中，WP培养基上毛状根的生长速度最快。光照抑制毛状根的生长。毛状根中5种黄酮类化合物的总量是愈伤组织中的1.5倍，其中甘草查尔酮含量是愈伤组织的15.5倍。
所建立的毛状根离体培养体系为通过毛状根培养大规模生产黄酮类化合物奠定了基础。
