Wang Xin, Xin Xiao-yan, Huang Yan-hong
Xi Jing Hospital, Fourth Military Medical University of Chinese PLA, Xi'an 710032, China.
Zhongguo Zhong Yao Za Zhi. 2006 Jun;31(11):901-4.
To investigate the regulative effect of genistein on the apoptosis of the xenografted tumors of human ovarian carcinoma HO-8910PM cell on the nude mice.
Human ovarian carcinoma HO-8910PM were cultured in vitro. The models of xenografted tumor were established by the transplantation of human ovarian carcinoma HO-8910PM on nude mice. The nude mice were randomly divided into four groups of three treatment groups (in which genistein were administered ip at 5, 25 and 50 mg x kg(-1) x d(-1), respectively, for 4 weeks) and one control group, the cell cycle and apoptosis of the xenografted tumors were measured by flow cytometry, the expression of bcl-2, Fas and FasL gene of xenografted tumors were determined by the immunohistochemistry and the morphology of tumor cells was observed by electron microscope.
The tumor weights of 50 mg x xkg(-1) genistein group were more lighter (P < 0.05) compared to those of control group. The tumor cells in Go-G1 phase were increased, at saml time the cells in S-phase were decreased (P < 0.01) after the treatment of 50 mg x kg(-1) genistein. In addition the apoptosis rate of the cell treated with 50 mg x kg(-1) genistein group was (15.14 +/- 2.27)%, which was significantly higher than that in the control group (3.12 +/- 1.12)% (P < 0.01). The expression of bcl-2 of the xenografted tumors was decreased and the expression of Fas was increased in 50 mg x kg(-1) genistein group, both showing a significant difference from those in control group (P < 0.05). The apoptosis of the tumor cells were found more under electron microscopy in 50 mg x kg(-1) genistein group than those in control group.
Genistein could significantly inhibite the proliferation and induce the apoptosis of the HO-8910PM cell of xenografted tumors by regulating the cell cycle and apoptoic gene in the nude mice.
探讨染料木黄酮对人卵巢癌HO - 8910PM细胞裸鼠移植瘤细胞凋亡的调控作用。
体外培养人卵巢癌HO - 8910PM细胞，将人卵巢癌HO - 8910PM接种于裸鼠建立移植瘤模型。将裸鼠随机分为三个治疗组（分别腹腔注射染料木黄酮5、25和50mg·kg⁻¹·d⁻¹，共4周）和一个对照组，采用流式细胞术检测移植瘤细胞周期及凋亡情况，免疫组织化学法检测移植瘤bcl - 2、Fas和FasL基因表达，电镜观察肿瘤细胞形态。
50mg·kg⁻¹染料木黄酮组肿瘤重量较对照组明显减轻（P < 0.05）。50mg·kg⁻¹染料木黄酮处理后，肿瘤细胞G₀ - G₁期细胞增多，S期细胞减少（P < 0.01）。此外，50mg·kg⁻¹染料木黄酮组细胞凋亡率为（15.14 ± 2.27）%，明显高于对照组（3.12 ± 1.12）%（P < 0.01）。50mg·kg⁻¹染料木黄酮组移植瘤bcl - 2表达降低，Fas表达增加，与对照组相比均有显著差异（P < 0.05）。电镜下可见50mg·kg⁻¹染料木黄酮组肿瘤细胞凋亡较对照组增多。
染料木黄酮可通过调控裸鼠移植瘤细胞周期及凋亡基因，显著抑制HO - 8910PM细胞增殖并诱导其凋亡。
