Cenciarelli C, Currier S J, Willingham M C, Thiebaut F, Germann U A, Rutherford A V, Gottesman M M, Barca S, Tombesi M, Morrone S, Santoni A, Mariani M, Ramoni C, Dupuis M L, Cianfriglia M
Laboratorio di Immunologia, Istituto Superiore di Sanità, Rome, Italy.
Int J Cancer. 1991 Feb 20;47(4):533-43. doi: 10.1002/ijc.2910470411.
We isolated an IgG2a murine monoclonal antibody (MAb) termed MAb57, specifically reactive with multi-drug-resistant (MDR) human cells. Its specificity toward the MDRI gene product (P-glycoprotein) has been demonstrated by the concordant segregation of the MAb57 epitope with the MDRI gene in interspecific mouse x human cell hybrids, and the reactivity of several different MDRI gene-expressing cells with MAb57, particularly insect cells acutely infected with a baculovirus encoding the MDRI gene. MAb57 can be used to detect, by flow cytometry, variations in the relative drug-resistance levels of several MDR KB and CEM cell variants. This immunological probe has also proven useful in selectively destroying MDR target cells in an antibody-dependent cell-mediated (ADCC) assay system as well as in detecting P-glycoprotein expression in normal and malignant tissues and cells.
我们分离出一种名为MAb57的IgG2a小鼠单克隆抗体，它能与多药耐药（MDR）人类细胞特异性反应。通过种间小鼠×人类细胞杂交中MAb57表位与MDRI基因的协同分离，以及几种不同的表达MDRI基因的细胞与MAb57的反应性，特别是被编码MDRI基因的杆状病毒急性感染的昆虫细胞，证明了它对MDRI基因产物（P-糖蛋白）的特异性。MAb57可用于通过流式细胞术检测几种MDR KB和CEM细胞变体相对耐药水平的变化。这种免疫探针在抗体依赖性细胞介导（ADCC）检测系统中选择性破坏MDR靶细胞以及检测正常和恶性组织及细胞中的P-糖蛋白表达方面也已证明是有用的。
