Expression of nicotinic acetylcholine receptor subunit alpha9 in type II vestibular hair cells of rats.

AIM

To explore the cell specific existence of alpha 9 AChR in the vestibular type II hair cells (VHC II) of rats.

METHODS

To detect the expression of alpha 9 AChR messenger RNA (mRNA) in the vestibular endorgans and single VHC II of rats by using the reverse transcription polymerase chain reaction (RT-PCR) technique and the single cell RT-PCR technique, respectively.

RESULTS

It was shown that alpha 9 AChR mRNA was detected in the vestibular endorgans. By using single-cell RT-PCR, mRNA encoding alpha 9 AChR was also detected in the VHC II of the rats. Sequence analysis of the PCR products confirmed identity to corresponding cDNA sequence in the predicted region.

CONCLUSION

We established a method which could effectively detect the cell specific expression of mRNA in an individual VHC. Present data confirm that alpha 9 AChR mRNA is expressed in the VHC II of rats and indicates that alpha 9 AChR may function as a mediator of efferent cholinergic signaling in mammalian VHC.