Hejnova Jana, Pages Delphine, Rusniok Christophe, Glaser Philippe, Sebo Peter, Buchrieser Carmen
Unité de Génomique des Microorganismes Pathogènes, Institut Pasteur, 28 Rue du Dr. Roux, F-75724 Paris, France.
Int J Med Microbiol. 2006 Dec;296(8):541-6. doi: 10.1016/j.ijmm.2006.06.007. Epub 2006 Oct 16.
Escherichia coli A0 34/86 (O83:K24:H31) is a commensal strain that has been used for prophylactic and therapeutic colonization of the intestine of newborn infants. To identify traits specific for E. coli A0 34/86, we used a minimal tiling set of 148 BAC clones of A0 34/86 genomic DNA, to construct restriction-digested BAC arrays. Hybridization with genomic DNA from four E. coli strains (CFT073; O157:H7; K12 and Nissle 1917) allowed selection of two BAC clones that were sequenced to identify A0 34/86-specific regions. Genes for the yersiniabactin siderophore system, several proteins homologous to Salmonella enterica serovar Typhimurium vitamin B12 synthesis proteins, as well as genes necessary for the degradation of propanediol, the pix fimbriae determinant and genes coding for a putative phosphoglycerate transport system present also on pathogenicity island V of E. coli strain 536 were all identified in E. coli A0 34/86. This comparative analysis underlines the important genome heterogeneity between E. coli strains.
大肠杆菌A0 34/86（O83:K24:H31）是一种共生菌株，已被用于新生儿肠道的预防性和治疗性定植。为了鉴定大肠杆菌A0 34/86的特异性特征，我们使用了一组由148个A0 34/86基因组DNA的BAC克隆组成的最小平铺集，构建了经酶切的BAC阵列。与来自四种大肠杆菌菌株（CFT073；O157:H7；K12和Nissle 1917）的基因组DNA杂交，筛选出两个进行测序的BAC克隆，以鉴定A0 34/86特异性区域。在大肠杆菌A0 34/86中鉴定出了耶尔森菌素铁载体系统的基因、几种与鼠伤寒沙门氏菌维生素B12合成蛋白同源的蛋白质，以及丙二醇降解所需的基因、pix菌毛决定簇和编码推定磷酸甘油酸转运系统的基因，这些基因也存在于大肠杆菌菌株536的致病岛V上。这种比较分析突显了大肠杆菌菌株之间重要的基因组异质性。
