Dunham Stephen P, Bruce Jennifer, Klein Dieter, Flynn J Norman, Golder Matthew C, MacDonald Susan, Jarrett Oswald, Neil James C
Retrovirus Research Laboratory, Institute of Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow, Bearsden Road, Glasgow G61 1QH, United Kingdom.
Vaccine. 2006 Nov 30;24(49-50):7095-108. doi: 10.1016/j.vaccine.2006.07.001. Epub 2006 Jul 18.
Protection against feline immunodeficiency virus (FIV) has been achieved using a variety of vaccines notably whole inactivated virus (WIV) and DNA. However protection against more virulent isolates, typical of those encountered in natural infections, has been difficult to achieve. In an attempt to improve protection against virulent FIV(GL8), we combined both DNA and WIV vaccines in a "prime-boost" approach. Thirty cats were divided into four groups receiving vaccinations and one unvaccinated control group. Following viral challenge, two vaccinated animals, one receiving DNA alone and one the prime-boost vaccine remained free of viraemia, whilst all controls became viraemic. Animals vaccinated with WIV showed apparent early enhancement of infection at 2 weeks post challenge (pc) with higher plasma viral RNA loads than control animals or cats immunised with DNA alone. Despite this, animals vaccinated with WIV or DNA alone showed significantly lower proviral loads in peripheral blood mononuclear cells and mesenteric lymph node cells, whilst those receiving the DNA-WIV prime-boost vaccine showed significantly lower proviral loads in PBMC, than control animals, at 35 weeks pc. Therefore both DNA and WIV vaccines conferred limited protection against viral challenge but the combination of WIV and DNA in a prime-boost approach appeared to offer no significant advantage over either vaccine alone.
使用多种疫苗，尤其是全灭活病毒（WIV）和DNA疫苗，已实现对猫免疫缺陷病毒（FIV）的防护。然而，要实现对自然感染中常见的更具毒性的分离株的防护却很困难。为了提高对毒性FIV（GL8）的防护效果，我们采用“初免-加强”策略将DNA疫苗和WIV疫苗联合使用。30只猫被分为四组接受疫苗接种，另有一组未接种作为对照组。在病毒攻击后，两只接种疫苗的动物，一只仅接受DNA疫苗，另一只接受初免-加强疫苗，未出现病毒血症，而所有对照组动物都出现了病毒血症。接种WIV疫苗的动物在攻击后2周（pc）时，血浆病毒RNA载量高于对照组动物或仅接种DNA疫苗的猫，显示出感染的早期明显增强。尽管如此，仅接种WIV或DNA疫苗的动物在外周血单核细胞和肠系膜淋巴结细胞中的前病毒载量显著较低，而接受DNA-WIV初免-加强疫苗的动物在pc 35周时，外周血单核细胞中的前病毒载量比对照组动物显著更低。因此，DNA疫苗和WIV疫苗对病毒攻击都提供了有限的防护，但WIV和DNA疫苗以初免-加强方式联合使用，相比单独使用任何一种疫苗似乎并没有显著优势。
