Hoile Rebecca, Yuen Marion, James Gregory, Gilbert Gwendolyn L
Forensic Counter Terrorism and Disaster Victim Identification Unit, NSW Police Headquarters, Level 5 Tower B, 1 Charles Street, Parramatta, New South Wales 2150, Australia.
Forensic Sci Int. 2007 Aug 24;171(1):1-4. doi: 10.1016/j.forsciint.2006.09.004. Epub 2006 Oct 17.
The aim of this study was to evaluate the accuracy and reliability of the rapid analyte measurement platform (RAMP) for presumptive identification of Bacillus anthracis spores. Test samples consisted of serial dilutions of spore preparations of several Bacillus species, including B. anthracis, which were tested, using the RAMP Anthrax test cartridge, according to the manufacturer's instructions. The fluorescence labelled antibody-antigen complexes were detected in the portable reader after 15 min following sample addition. Dilutions of common environmental and household powders were also tested to identify possible false positive results. B. anthracis spores were identified reliably in test samples containing more than 6000 spores. The test kits were highly specific, showing no cross reactivity with other Bacillus species or any environmental powders tested. The RAMP system for detection of B. anthracis spores, from environmental samples, showed consistent results under a variety of analytical conditions, enabling the trained user to provide a rapid, accurate preliminary risk assessment of a suspected bioterrorism incident.
本研究的目的是评估快速分析物测量平台（RAMP）对炭疽芽孢杆菌孢子进行推定鉴定的准确性和可靠性。测试样品包括几种芽孢杆菌（包括炭疽芽孢杆菌）孢子制剂的系列稀释液，按照制造商的说明，使用RAMP炭疽检测试剂盒对其进行测试。加样15分钟后，在便携式读数器中检测荧光标记的抗体 - 抗原复合物。还对常见环境和家用粉末的稀释液进行了测试，以确定可能的假阳性结果。在含有超过6000个孢子的测试样品中能够可靠地鉴定出炭疽芽孢杆菌孢子。该测试试剂盒具有高度特异性，与其他芽孢杆菌物种或任何测试的环境粉末均无交叉反应。用于从环境样品中检测炭疽芽孢杆菌孢子的RAMP系统在各种分析条件下均显示出一致的结果，使经过培训的用户能够对疑似生物恐怖主义事件进行快速、准确的初步风险评估。
