Matsuda M, Mayer B J, Hanafusa H
Rockefeller University, New York, New York 10021.
Mol Cell Biol. 1991 Mar;11(3):1607-13. doi: 10.1128/mcb.11.3.1607-1613.1991.
The oncogene product of the avian sarcoma virus CT10, P47gag-crk, contains the SH2, SH2', and SH3 domains and binds proteins in a phosphotyrosine (ptyr)-dependent manner. In this study, we have determined the region of P47gag-crk essential for binding to ptyr-containing proteins. Mutant P47gag-crk proteins expressed in Escherichia coli that have the intact SH2 and SH2' regions retained the capacity to bind ptyr-containing proteins obtained from cells transformed by crk and src. The deletion of SH2 resulted in the loss of binding activity. Other mutants that have altered SH2 or SH2' bound few, if any, of the ptyr-containing proteins. Those mutants that bound ptyr-containing proteins associated with tyrosine kinase activity. We also found that polypeptides containing SH2, SH2', and SH3 of p60v-src and p60c-src associated with ptyr-containing proteins from crk-transformed cells. Thus, the SH2 and SH2' domains of P47gag-crk are responsible for their binding to ptyr-containing proteins.
禽肉瘤病毒CT10的癌基因产物P47gag-crk包含SH2、SH2'和SH3结构域，并以磷酸酪氨酸（ptyr）依赖的方式结合蛋白质。在本研究中，我们确定了P47gag-crk中与含ptyr蛋白质结合所必需的区域。在大肠杆菌中表达的具有完整SH2和SH2'区域的突变型P47gag-crk蛋白保留了与从经crk和src转化的细胞中获得的含ptyr蛋白质结合的能力。SH2的缺失导致结合活性丧失。其他改变了SH2或SH2'的突变体几乎不与含ptyr的蛋白质结合（如果有的话）。那些与含ptyr蛋白质结合的突变体与酪氨酸激酶活性相关。我们还发现，包含p60v-src和p60c-src的SH2、SH2'和SH3的多肽与来自crk转化细胞的含ptyr蛋白质相关。因此，P47gag-crk的SH2和SH2'结构域负责其与含ptyr蛋白质的结合。
