Ozturk Kayhan, Yaman Huseyin, Cihat Avunduk Mustafa, Arbag Hamdi, Keles Bahar, Uyar Yavuz
Department of Otolaryngology, Meram Medical Faculty, Selçuk University, Konya, Turkey.
Acta Otolaryngol. 2006 Dec;126(11):1158-63. doi: 10.1080/00016480600678797.
Our results support the proposition that hyaluronic acid (HA) provides a moist wound-healing environment to aid in the healing process of tympanic membrane perforation. A single MeroGel administration can be effective as well as daily topical HA application in the treatment of tympanic membrane perforations. A single application of esterified HA may be more suitable for patients and also for otolaryngologists.
The purpose of the present study was to evaluate the effectiveness of a single MeroGel application on traumatic tympanic membrane perforations in rats.
The posterior quadrant of the tympanic membranes in both ears of 24 male pathogen-free Sprague-Dawley rats was perforated with a 20-gauge needle. Subjects were divided into two groups: MeroGel and daily topical HA-treated groups. All subjects were sacrificed and histopathological examinations of the tympanic bullas were carried out.
Perforations of controls, and MeroGel- and daily HA-treated groups closed in 17/24 (70.8%), 11/12 (91.7%), and 12/12 (100%) ears, respectively. There was a significant difference between control and MeroGel-treated groups, and also between control and daily topical HA-treated groups for the presence of vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), lymphocytes and collagen fibrils (p<0.05), whereas there was no significant difference between MeroGel- and daily topical HA-treated groups (p>0.05).
我们的结果支持以下观点，即透明质酸（HA）可提供一个湿润的伤口愈合环境，以辅助鼓膜穿孔的愈合过程。在鼓膜穿孔的治疗中，单次应用MeroGel与每日局部应用HA一样有效。单次应用酯化HA可能对患者以及耳鼻喉科医生来说更为合适。
本研究的目的是评估单次应用MeroGel对大鼠外伤性鼓膜穿孔的有效性。
用20号针头在24只雄性无特定病原体的Sprague-Dawley大鼠双耳鼓膜的后象限穿孔。将实验对象分为两组：MeroGel组和每日局部应用HA治疗组。所有实验对象均处死后对鼓泡进行组织病理学检查。
对照组、MeroGel治疗组和每日HA治疗组的穿孔分别在17/24（70.8%）、11/12（91.7%）和12/12（100%）的耳中闭合。在血管内皮生长因子（VEGF）、成纤维细胞生长因子（FGF）、淋巴细胞和胶原纤维的存在方面，对照组与MeroGel治疗组之间以及对照组与每日局部应用HA治疗组之间存在显著差异（p<0.05），而MeroGel治疗组与每日局部应用HA治疗组之间无显著差异（p>0.05）。
