Davies C A, Jeziorska M, Freemont A J, Herrick A L
Rheumatic Diseases Centre, Clinical Sciences Building, Hope Hospital, Eccles Old Road, Salford M6 8HD, UK.
Rheumatology (Oxford). 2006 Nov;45(11):1349-55. doi: 10.1093/rheumatology/kei277.
Our aim was to evaluate (i) whether the bone matrix proteins osteonectin and matrix gamma-carboxyglutamic acid protein (MGP) are up-regulated in skin biopsies from patients with systemic sclerosis (SSc) and (ii) whether there is differential expression between patients with and without dermal calcinosis, a distressing and debilitating complication of SSc.
Skin punch biopsies were taken from the forearms of 38 SSc patients with the limited cutaneous subtype of SSc [17 without calcinosis (lcSSc) and 21 with calcinosis (lcSScCal)] and from 11 healthy control subjects. Immunohistochemistry was performed with antibodies to osteonectin and MGP. Staining was assessed semiquantitatively in the microvascular endothelium and in dermal fibroblasts. The Kruskal-Wallis one-way ANOVA was used to compare the data between patient groups.
Both lcSSc and lcSScCal groups showed a statistically significant increase in the percentage of microvessels with osteonectin-positive endothelial cells (EC) (especially the lcSScCal group), whereas lcSScCal alone showed an increase in the percentage of microvessels with MGP-positive EC when compared with controls. In both SSc groups, the percentage of osteonectin and MGP-stained fibroblasts was increased in the reticular dermis (for osteonectin this was more marked in the lcSScCal group). In the papillary dermis, the percentage of osteonectin-stained fibroblasts was increased in both SSc groups but the lcSScCal group alone had a higher percentage of MGP-stained fibroblasts.
When compared with controls, protein expression of osteonectin and MGP was greater in SSc patients generally, and osteonectin expression was significantly higher in EC and fibroblasts of the lcSScCal patients than the lcSSc patients without calcinosis.
我们的目的是评估（i）骨基质蛋白骨连接蛋白和基质γ-羧基谷氨酸蛋白（MGP）在系统性硬化症（SSc）患者的皮肤活检组织中是否上调，以及（ii）有和没有皮肤钙化（SSc的一种令人痛苦且使人衰弱的并发症）的患者之间是否存在差异表达。
从38例局限性皮肤型SSc患者（17例无皮肤钙化（lcSSc）和21例有皮肤钙化（lcSScCal））的前臂以及11名健康对照者身上获取皮肤打孔活检组织。使用抗骨连接蛋白和MGP的抗体进行免疫组织化学。在微血管内皮细胞和真皮成纤维细胞中对染色进行半定量评估。使用Kruskal-Wallis单向方差分析比较患者组之间的数据。
lcSSc组和lcSScCal组中骨连接蛋白阳性内皮细胞（EC）的微血管百分比均有统计学显著增加（尤其是lcSScCal组），而与对照组相比，仅lcSScCal组中MGP阳性EC的微血管百分比增加。在两个SSc组中，网状真皮中骨连接蛋白和MGP染色的成纤维细胞百分比均增加（对于骨连接蛋白，在lcSScCal组中更明显）。在乳头真皮中，两个SSc组中骨连接蛋白染色的成纤维细胞百分比均增加，但仅lcSScCal组中MGP染色的成纤维细胞百分比更高。
与对照组相比，SSc患者总体上骨连接蛋白和MGP的蛋白表达更高，并且lcSScCal患者的EC和成纤维细胞中骨连接蛋白的表达明显高于无皮肤钙化的lcSSc患者。
