Hawthorne J N, Bleasdale J E
Mol Cell Biochem. 1975 Aug 30;8(2):83-7. doi: 10.1007/BF02116236.
Synaptosomes isolated from guinea pig brain cortex were stimulated electrically in a medium containing [32P]-orthophosphate. The electrical stimulation caused increased labelling of phosphatidic acid in a synaptic vesicle fraction prepared by osmotic shock of the incubated synaptosomes. Electrical stimulation also provokes transmitter release from the synaptosomes. Both increased phosphatidate labelling and transmitter release required calcium ions in the medium. The effects are discussed in relation to earlier work with acetylcholine and the possible involvement of membrane phosphatidic acid in transmitter release by exocytosis.
从豚鼠大脑皮层分离出的突触体在含有[32P] - 正磷酸盐的培养基中进行电刺激。电刺激导致通过对孵育的突触体进行渗透压休克制备的突触小泡部分中磷脂酸的标记增加。电刺激还引发突触体释放神经递质。磷脂酸标记增加和神经递质释放都需要培养基中的钙离子。结合早期关于乙酰胆碱的研究以及膜磷脂酸可能通过胞吐作用参与神经递质释放的情况对这些效应进行了讨论。
