Konno S, Kobayashi H, Kawazoe T, Hiyama T, Tajima H, Minoguchi K, Hoshino H, Okazawa A, Adachi M, Takahashi T
First Department of Internal Medicine School of Medicine, Showa University.
Arerugi. 1990 Nov;39(11):1499-508.
We examined lung tissues in a guinea pig model actively sensitized by inhalation of aerosolized ovalbumin (OA) and found reproducible late bronchial responses (LBR) 7 h and 24 h after OA challenge. Light microscopic examination revealed bronchoconstriction, damage to the epithelium, eosinophil infiltration in both the epithelium and subepithelium, retention of mucous in the pulmonary bronchial lumens and a decrease in the mucous content of Periodic Acid-Schiff (PAS) stain positive cells in the bronchial epithelium in LBR. An increase in the number of PAS-stain positive cells of in the bronchial epithelium was also observed in LBR. Electron microscopic examination revealed eosinophil migration around mast cell at 7 hs after OA challenge. Two different mechanisms of degranulation of eosinophil specific granules were observed in LBR. In one mechanism, the granule core changes after its matrix has changed. In the other mechanism, the granules were exocytosed as a whole, accompanied by the cell membrane and densely clumped chromatin, suggesting burst of eosinophil.
我们在通过雾化吸入卵清蛋白（OA）主动致敏的豚鼠模型中检查了肺组织，发现在OA激发后7小时和24小时出现了可重复的迟发性支气管反应（LBR）。光学显微镜检查显示，LBR中存在支气管收缩、上皮损伤、上皮和上皮下嗜酸性粒细胞浸润、肺支气管腔内黏液潴留以及支气管上皮中过碘酸希夫（PAS）染色阳性细胞黏液含量减少。在LBR中还观察到支气管上皮中PAS染色阳性细胞数量增加。电子显微镜检查显示，OA激发后7小时嗜酸性粒细胞围绕肥大细胞迁移。在LBR中观察到嗜酸性粒细胞特异性颗粒脱颗粒的两种不同机制。一种机制是，颗粒基质改变后颗粒核心发生变化。另一种机制是，颗粒作为一个整体被胞吐，伴随着细胞膜和密集聚集的染色质，提示嗜酸性粒细胞破裂。
