蛛网膜下腔出血对兔基底动脉中5-羟色胺摄取及代谢的影响。

Effect of subarachnoid hemorrhage on serotonin uptake and metabolism in rabbit basilar artery.

作者信息

Tanaka Y, Kassell N F, Machi T, Dougherty D A

机构信息

Department of Neurological Surgery, University of Virginia Health Sciences Center, Charlottesville 22908.

出版信息

J Cereb Blood Flow Metab. 1991 Mar;11(2):342-6. doi: 10.1038/jcbfm.1991.68.

DOI:10.1038/jcbfm.1991.68

PMID:1705255

Abstract

The effects of subarachnoid hemorrhage (SAH) on neuronal uptake and metabolism of serotonin (5-HT) in the rabbit basilar artery were examined. Extracted 3H-amines from the isolated arteries after incubation with [3H]5-HT were separated by column chromatography. Radioactivity of 5-HT and 5-hydroxyindoleacetic acid was, respectively, 52.7 +/- 13.9 and 22.9 +/- 5.4 x 10(2) dpm/mg tissue in the control group (n = 8); 32 and 18% of control after denervation (n = 6); 99 and 12% of control after treatment with pargyline (n = 7); and 65 and 76% of control after SAH (n = 7). These results suggest that the neuronal uptake of 5-HT is impaired by SAH, although monoamine oxidase activity is relatively preserved.

摘要

研究了蛛网膜下腔出血（SAH）对兔基底动脉中5-羟色胺（5-HT）的神经元摄取及代谢的影响。将离体动脉与[3H]5-HT孵育后，从其中提取的3H-胺通过柱色谱法进行分离。对照组（n = 8）中5-HT和5-羟吲哚乙酸的放射性分别为52.7±13.9和22.9±5.4×10(2) dpm/mg组织；去神经支配后（n = 6）分别为对照组的32%和18%；帕吉林治疗后（n = 7）分别为对照组的99%和12%；SAH后（n = 7）分别为对照组的65%和76%。这些结果表明，尽管单胺氧化酶活性相对保留，但SAH会损害5-HT的神经元摄取。