Nozal Leonor, Arce Lourdes, Simonet Bartolomé M, Ríos Angel, Valcárcel Miguel
Department of Analytical Chemistry, University of Córdoba, Campus de Rabanales, Córdoba, Spain.
Electrophoresis. 2006 Nov;27(22):4439-45. doi: 10.1002/elps.200600077.
A new hexane-in-water microemulsion was investigated as buffer in microemulsion EKC (MEEKC). At difference with other microemulsions, the addition of cosurfactant was not necessary to stabilize the microemulsion. The proposed microemulsion was successfully used to achieve electrophoretic separation of seven antibiotics including nitroimidazoles, cephapirin and tetracyclines. Selectivity and separation efficiency achieved in MEEKC were compared with MEKC. MEEKC technique proved to be more efficient than MEKC for performing the separation of the analytes and the presence of microemulsions was found to be critical to achieve the separation of tetracyclines. The proposed microemulsion also points out that solvents with high volatility, such as hexane, can be stabilized and used as a microemulsion of SDS.
研究了一种新型水包己烷微乳液作为微乳液电动色谱（MEEKC）中的缓冲液。与其他微乳液不同的是，该微乳液无需添加助表面活性剂来保持稳定。所提出的微乳液成功用于实现包括硝基咪唑、头孢匹林和四环素在内的七种抗生素的电泳分离。将MEEKC中实现的选择性和分离效率与MEKC进行了比较。事实证明，MEEKC技术在分离分析物方面比MEKC更有效，并且发现微乳液的存在对于实现四环素的分离至关重要。所提出的微乳液还表明，具有高挥发性的溶剂，如己烷，可以被稳定化并用作SDS微乳液。
