Jiroutova Alena, Slavkovsky Rastislav, Cermakova Martina, Majdiakova Lenka, Hanovcova Irena, Bolehovska Radka, Hajzlerova Milena, Radilova Hana, Ruszova Ema, Kanta Jiri
Charles University in Prague, Faculty of Medicine in Hradec Kralove, Department of Medical Biochemistry, Simkova 870, 500 38 Hradec Kralove, Czech Republic.
Exp Toxicol Pathol. 2007 Jan;58(4):263-73. doi: 10.1016/j.etp.2006.06.005. Epub 2006 Oct 23.
Hepatic stellate cells (HSC) and liver myofibroblasts (MFB) are two cell populations most likely responsible for the synthesis of most connective tissue components in fibrotic liver. They differ in their origin and location, and possibly in patterns of gene expression. Normal and carbon tetrachloride-cirrhotic livers from rats were used to isolate HSC. Liver was perfused with pronase and collagenase solutions, followed by centrifugation of the cell suspension on a density gradient. HSC were quiescent 2 days after plating on plastic but they became activated after another 5 days in culture. When the culture was passaged 5 times, its character changed profoundly as HSC were replaced by MFB. Microarray analysis was used to determine gene expression in quiescent HSC, activated HSC and MFB. The expression of 49 genes coding for connective tissue proteins, proteoglycans, metalloproteinases and their inhibitors, growth factors and cellular markers was determined. The pattern of gene expression changed during HSC activation and there were distinct differences between HSC and MFB. Little difference between normal cells and cells isolated from cirrhotic liver was found.
肝星状细胞（HSC）和肝肌成纤维细胞（MFB）是最有可能负责纤维化肝脏中大多数结缔组织成分合成的两种细胞群体。它们在起源和位置上有所不同，基因表达模式可能也存在差异。使用来自大鼠的正常肝脏和四氯化碳诱导肝硬化的肝脏来分离HSC。用链霉蛋白酶和胶原酶溶液灌注肝脏，然后将细胞悬液在密度梯度上进行离心。接种到塑料培养皿上2天后HSC处于静止状态，但在培养5天后它们被激活。当培养物传代5次时，其特性发生了深刻变化，因为HSC被MFB取代了。利用基因芯片分析来确定静止HSC、激活的HSC和MFB中的基因表达。测定了49个编码结缔组织蛋白、蛋白聚糖、金属蛋白酶及其抑制剂、生长因子和细胞标志物的基因的表达。在HSC激活过程中基因表达模式发生了变化，并且HSC和MFB之间存在明显差异。未发现正常细胞与从肝硬化肝脏分离的细胞之间有显著差异。
