Ui-Tei Kumiko, Naito Yuki, Saigo Kaoru
Department of Biophysics and Biochemistry, Graduate School of Science and Undergraduate Program for Bioinformatics and Systems Biology, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
J Biomed Biotechnol. 2006;2006(4):65052. doi: 10.1155/JBB/2006/65052.
Short interfering RNAs (siRNAs) are widely used to bring about RNA interference (RNAi) in mammalian cells. Numerous siRNAs may be designed for any target gene though most of which would be incapable of efficiently inducing mammalian RNAi. Certain highly functional siRNAs designed for knockout of a particular gene may render unrelated endogenous genes nonfunctional. These major bottlenecks should be properly eliminated when RNAi technologies are employed for any experiment in mammalian functional genomics. This paper thus presents essential notes and findings regarding the proper choice of siRNA-sequence selection algorithms and web-based online software systems.
小干扰RNA（siRNAs）被广泛用于在哺乳动物细胞中引发RNA干扰（RNAi）。针对任何靶基因都可以设计出众多的siRNAs，不过其中大多数无法有效诱导哺乳动物RNAi。为敲除特定基因而设计的某些高效功能siRNAs可能会使不相关的内源性基因失去功能。当将RNAi技术用于哺乳动物功能基因组学的任何实验时，这些主要瓶颈都应妥善消除。因此，本文介绍了有关siRNA序列选择算法和基于网络的在线软件系统正确选择的重要注意事项和研究结果。
