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大鼠肝癌细胞核仁DNA。2. 核仁DNA分析。

Rat hepatoma cells nucleolar DNA. 2. Analysis of nucleolar DNA.

作者信息

Amalric F, Zalta J P

出版信息

Nucleic Acids Res. 1975 Aug;2(8):1305-20. doi: 10.1093/nar/2.8.1305.

DOI:10.1093/nar/2.8.1305
PMID:170593
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC344383/
Abstract

The analysis by CsCl density gradient of nucleolar DNA has revealed that the 1.700 g/cm3 main component can be subdivided in three subcomponents with buoyant densities of 1.707 g/cm3, 1.700 g/cm3, 1.690 g/cm3. The 1.707 g/cm3 and 1.690 g/cm3 components contain all the repetitive sequences which comprise 15 % of the total nucleolar DNA. The ribosomal cistrons are found in components having buoyant density between 1.707 g/cm3 and 1.725 g/gm3. Sodium-p-aminosalicylate-DNA interactions have revealed that only the 1.700 g/cm3 fraction has a destabilized secondary structure. The possible localization of these different fractions on peri and intranucleolar fractions is discussed.

摘要

通过氯化铯密度梯度对核仁DNA进行分析发现，1.700克/立方厘米的主要成分可细分为三个子成分，其浮力密度分别为1.707克/立方厘米、1.700克/立方厘米、1.690克/立方厘米。1.707克/立方厘米和1.690克/立方厘米的成分包含了所有重复序列，这些重复序列占核仁DNA总量的15%。核糖体顺反子存在于浮力密度在1.707克/立方厘米至1.725克/立方厘米之间的成分中。对氨基水杨酸钠与DNA的相互作用表明，只有1.700克/立方厘米的部分具有不稳定的二级结构。本文讨论了这些不同部分在核仁周围和核仁内部分的可能定位。

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本文引用的文献

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