Koch A E, Burrows J C, Haines G K, Carlos T M, Harlan J M, Leibovich S J
Department of Medicine, Northwestern University Medical School, Chicago.
Lab Invest. 1991 Mar;64(3):313-20.
Leukocyte adhesion to endothelium plays an important role in the development and perpetuation of chronic inflammatory diseases such as rheumatoid arthritis (RA). In order to help define the role of adhesion molecules in arthritic disorders, we have studied the expression of CD11c, endothelial leukocyte adhesion molecule-1 (ELAM-1), vascular cell adhesion molecule-1 (VCAM-1), and intercellular adhesion molecule-1 in synovial tissues from patients with RA and osteoarthritis (OA) by immunohistochemistry. CD11c is expressed predominantly on macrophages deep within RA and OA synovial tissues, as well as on some synovial tissue lining cells. ELAM-1 has endothelial reactivity, being present mainly on venules and capillaries and staining more blood vessels in RA than OA. VCAM-1 is present predominantly on synovial tissue macrophages and, to a lesser degree, on synovial tissue endothelial cells of venules, capillaries, and arterioles in both RA and OA. Like ELAM-1, VCAM-1 appears to be present more often on endothelial cells in RA than in OA tissues. VCAM-1 is present on macrophages isolated from RA synovium as well as macrophages in situ. Intercellular adhesion molecule-1 is more broadly distributed than the other adhesion molecules, being found on endothelium, macrophages, some fibroblasts, and some lymphocytes in both RA and OA tissues. This study shows that ELAM-1, a molecule that was previously thought to be important mainly in acute inflammatory reactions, is also found in RA, a chronic inflammatory disease, as well as in OA. Thus, ELAM-1 as well as VCAM-1 and intercellular adhesion molecule-1 may be involved in mediating the leukocyte traffic into RA and OA synovium.
白细胞与内皮细胞的黏附在类风湿关节炎（RA）等慢性炎症性疾病的发生和持续发展过程中起着重要作用。为了明确黏附分子在关节炎性疾病中的作用，我们通过免疫组织化学研究了RA和骨关节炎（OA）患者滑膜组织中CD11c、内皮白细胞黏附分子-1（ELAM-1）、血管细胞黏附分子-1（VCAM-1）和细胞间黏附分子-1的表达情况。CD11c主要表达于RA和OA滑膜组织深部的巨噬细胞以及一些滑膜组织衬里细胞上。ELAM-1具有内皮细胞反应性，主要存在于小静脉和毛细血管上，且RA中染色的血管比OA更多。VCAM-1主要存在于滑膜组织巨噬细胞上，在RA和OA的小静脉、毛细血管和小动脉的滑膜组织内皮细胞上也有少量表达。与ELAM-1一样，VCAM-1在RA的内皮细胞上出现的频率似乎比OA组织更高。VCAM-1存在于从RA滑膜分离出的巨噬细胞以及原位巨噬细胞上。细胞间黏附分子-1的分布比其他黏附分子更广泛，在RA和OA组织的内皮细胞、巨噬细胞、一些成纤维细胞和一些淋巴细胞上均有发现。这项研究表明，ELAM-1这种先前被认为主要在急性炎症反应中起重要作用的分子，也存在于慢性炎症性疾病RA以及OA中。因此，ELAM-1以及VCAM-1和细胞间黏附分子-1可能参与介导白细胞向RA和OA滑膜的迁移。
