Elling Ulrich, Klasen Christian, Eisenberger Tobias, Anlag Katrin, Treier Mathias
Developmental Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
Proc Natl Acad Sci U S A. 2006 Oct 31;103(44):16319-24. doi: 10.1073/pnas.0607884103. Epub 2006 Oct 23.
Sall4 is a mammalian Spalt transcription factor expressed by cells of the early embryo and germ cells, an expression pattern similar to that of both Oct4 and Sox2, which play essential roles during early murine development. We show that the activity of Sall4 is cell-autonomously required for the development of the epiblast and primitive endoderm from the inner cell mass. Furthermore, no embryonic or extraembryonic endoderm stem cell lines could be established from Sall4-deficient blastocysts. In contrast, neither the development of the trophoblast lineage nor the ability to generate trophoblast cell lines from murine blastocysts was impaired in the absence of Sall4. These data establish Sall4 as an essential transcription factor required for the early development of inner cell mass-derived cell lineages.
Sall4是一种哺乳动物的Spalt转录因子，由早期胚胎细胞和生殖细胞表达，其表达模式与Oct4和Sox2相似，它们在小鼠早期发育过程中发挥着重要作用。我们发现，从内细胞团发育形成上胚层和原始内胚层，Sall4的活性是细胞自主所需的。此外，无法从Sall4缺陷型囊胚中建立胚胎或胚外内胚层干细胞系。相比之下，在没有Sall4的情况下，滋养层谱系的发育以及从小鼠囊胚中产生滋养层细胞系的能力均未受损。这些数据表明，Sall4是内细胞团来源的细胞谱系早期发育所需的一种重要转录因子。
