使用针对重组核蛋白的单克隆抗体的马尔堡病毒核蛋白捕获酶联免疫吸附测定：检测天然马尔堡病毒

Marburgvirus nucleoprotein-capture enzyme-linked immunosorbent assay using monoclonal antibodies to recombinant nucleoprotein: detection of authentic Marburgvirus.

作者信息

Saijo Masayuki, Georges-Courbot Marie-Claude, Fukushi Shuetsu, Mizutani Tetsuya, Philippe Marianneau, Georges Alain-Jean, Kurane Ichiro, Morikawa Shigeru

机构信息

Department of Virology 1, National Institute of Infectious Diseases, Tokyo 208-0011, Japan.

出版信息

Jpn J Infect Dis. 2006 Oct;59(5):323-5.

PMID:17060700

Abstract

There have recently been large outbreaks of Marburg hemorrhagic fever (MHF) caused by Marburgvirus (MARV) in the Democratic Republic of Congo and Angola. The development of reliable diagnostic systems for MHF is urgently needed. An antigen-capture enzyme-linked immunosorbent assay (Ag-capture ELISA) using either of the two monoclonal antibodies (2A7 and 2H6) produced by immunizing mice with recombinant nucleoprotein of MARV was described (Journal of Medical Virology, 76, 111-118, 2005). In the present study, it was revealed that the Ag-capture ELISA specifically detected authentic MARV antigen and that the sensitivity of the Ag-capture ELISA was at a level similar to that of reverse-transcription polymerase chain reaction. These results suggest that the Ag-capture ELISA using the monoclonal antibodies, 2A7 and 2H6, is applicable to the diagnosis of MHF.

摘要

最近，刚果民主共和国和安哥拉爆发了由马尔堡病毒（MARV）引起的大规模马尔堡出血热（MHF）疫情。迫切需要开发可靠的MHF诊断系统。此前已报道了一种抗原捕获酶联免疫吸附测定（Ag-capture ELISA），该方法使用通过用MARV重组核蛋白免疫小鼠产生的两种单克隆抗体（2A7和2H6）中的任意一种（《医学病毒学杂志》，76卷，111 - 118页，2005年）。在本研究中，发现Ag-capture ELISA能特异性检测到真实的MARV抗原，且该方法的灵敏度与逆转录聚合酶链反应相当。这些结果表明，使用单克隆抗体2A7和2H6的Ag-capture ELISA可用于MHF的诊断。