Rothbauer Ulrich, Zolghadr Kourosh, Tillib Sergei, Nowak Danny, Schermelleh Lothar, Gahl Anja, Backmann Natalija, Conrath Katja, Muyldermans Serge, Cardoso M Cristina, Leonhardt Heinrich
Ludwig Maximilians University Munich, Department of Biology II, Grosshaderner Str. 2, 82152 Planegg-Martinsried, Germany.
Nat Methods. 2006 Nov;3(11):887-9. doi: 10.1038/nmeth953.
We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion.
我们将骆驼科动物重链抗体的表位识别片段与荧光蛋白融合，以生成可在活细胞中表达的具有荧光、能结合抗原的纳米抗体（染色体抗体）。我们证明，染色体抗体能够在整个S期和有丝分裂过程中识别并追踪不同亚细胞区室中的抗原。由于潜在地任何抗原结构都可以通过这种方式在活细胞中被靶向和追踪，染色体抗体应该能够实现新的功能研究。
