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从猪分离的肠球菌菌株对胃肠道的抗性及对大肠杆菌K88的拮抗作用。

Resistance of Enterococcus strains isolated from pigs to gastrointestinal tract and antagonistic effect against Escherichia coli K88.

作者信息

García-Galaz Alfonso, Pérez-Morales Rosalva, Díaz-Cinco Martha, Acedo-Félix Evelia

机构信息

Centro de Investigación en Alimentación y Desarrollo, Sonora, México.

出版信息

Rev Latinoam Microbiol. 2004 Jan-Jun;46(1-2):5-11.

Abstract

The intestinal flora plays an important role in health and wellbeing of different organisms. Indigenous microflora can be innocuous or pathogenic. Consumption of food supplemented with beneficial microorganisms as probiotics provides a good health state and this can be maintained and recovered. Currently, probiotic strains of Bifidobacterium and Lactobacillus are widely used in humans as well as animals. Swine industry would benefit with the application of probiotics, mainly to overcome diarrheal diseases produced by different causes, as a pathogenic E. coli K88. The aim of this work was to isolate strains of Enterococcus from gastrointestinal tract of pigs to use them as probiotic. Two strains of E. faecalis, 2 of E. mundii and 7 of E. faecium were isolated with characteristics of resistance to acid pH, tolerance to biliary salts and a high antagonistic activity (>80%) against E. coli K88. Based on their characteristics and species affinity, we believe that these strains could be administered to pigs as a probiotic.

摘要

肠道菌群在不同生物体的健康和福祉中发挥着重要作用。本土微生物群可能无害,也可能致病。食用添加有益微生物(即益生菌)的食物可使身体处于良好健康状态,且这种状态能够得以维持和恢复。目前,双歧杆菌和乳酸杆菌的益生菌菌株在人类和动物中都被广泛使用。养猪业将从益生菌的应用中受益,主要用于克服由不同原因引起的腹泻疾病,比如致病性大肠杆菌K88。这项工作的目的是从猪的胃肠道中分离肠球菌菌株,以将其用作益生菌。分离出了两株粪肠球菌、两株蒙氏肠球菌和七株屎肠球菌,它们具有耐酸性pH值、耐受胆盐的特性,并且对大肠杆菌K88具有较高的拮抗活性(>80%)。基于它们的特性和种属亲缘关系,我们认为这些菌株可以作为益生菌应用于猪。

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