Beyer H S, Stanley M
Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota.
Biochem Int. 1990 Nov;22(3):405-10.
Tumor necrosis factor-alpha (TNF) has been reported to increase DNA synthesis in normal rat liver. Therefore, we examined the effects of TNF on rat liver regeneration. TNF, 1.5 micrograms ip every 4 h for 5 d, significantly increased hepatic DNA and RNA contents of regenerating and sham operated livers by up to 45%. Mitotic figures in sham operated liver, usually rare, were increased substantially by TNF. ODC mRNA content and enzyme activity were increased in regenerating liver, and were further increased by TNF. These data indicate that TNF, although not specific for regenerating liver, is a potent stimulus for hepatocyte DNA synthesis and mitosis.
据报道，肿瘤坏死因子-α（TNF）可增加正常大鼠肝脏中的DNA合成。因此，我们研究了TNF对大鼠肝脏再生的影响。每4小时腹腔注射1.5微克TNF，持续5天，可使再生肝脏和假手术肝脏的肝DNA和RNA含量显著增加，增幅高达45%。假手术肝脏中通常罕见的有丝分裂象，因TNF而大幅增加。再生肝脏中鸟氨酸脱羧酶（ODC）mRNA含量和酶活性增加，且因TNF进一步增加。这些数据表明，TNF虽然对再生肝脏无特异性，但却是肝细胞DNA合成和有丝分裂的有效刺激因子。
