Bikker Floris J, Kaman-van Zanten Wendy E, de Vries-van de Ruit Anne-Marij B C, Voskamp-Visser Ingrid, van Hooft Peter A V, Mars-Groenendijk Roos H, de Visser Peter C, Noort Daan
TNO Defence, Security and Safety, PO Box 45, 2280 AA Rijswijk, the Netherlands.
Chem Biol Drug Des. 2006 Sep;68(3):148-53. doi: 10.1111/j.1747-0285.2006.00424.x.
Drosocin is a 19-mer, cationic antimicrobial peptide from Drosophila melanogaster. The aim of the study was to examine the antibacterial spectrum of unglycosylated drosocin analogues. Furthermore, the amino acid sequence of DnaK, drosocin's intracellular target, from susceptible species was aligned and studied for sequence homology. From this a panel of 31 bacterial strains, including Salmonella strains with truncated lipopolysaccharide structures, was tested for susceptibility towards three drosocin analogues. Available bacterial DnaK amino acid sequences were retrieved from the ExPASy proteomics server of the Swiss Institute of Bioinformatics studied for sequence homology. Seventeen of the 31 strains tested were susceptible for the drosocin analogues. Minimal inhibitory concentration values against mainly Gram-negative bacteria ranged from 3.1 to 100 microm. With the exception of Micrococcus luteus and Xanthomonas campestris all drosocin analogue-susceptible strains were Enterobacteriaceae showing a high DnaK amino acid sequence homology.
果蝇抗菌肽是一种来自黑腹果蝇的由19个氨基酸组成的阳离子抗菌肽。本研究的目的是检测未糖基化的果蝇抗菌肽类似物的抗菌谱。此外，对来自敏感物种的果蝇抗菌肽的细胞内靶点DnaK的氨基酸序列进行比对，并研究其序列同源性。据此，对包括具有截短脂多糖结构的沙门氏菌菌株在内的31株细菌进行了测试，以检测它们对三种果蝇抗菌肽类似物的敏感性。从瑞士生物信息学研究所的ExPASy蛋白质组学服务器中检索了可用的细菌DnaK氨基酸序列，并研究了其序列同源性。在测试的31株菌株中，有17株对果蝇抗菌肽类似物敏感。对主要革兰氏阴性菌的最低抑菌浓度值范围为3.1至100微摩尔。除藤黄微球菌和野油菜黄单胞菌外
