Vancraeynest D, Haesebrouck F, Deplano A, Denis O, Godard C, Wildemauwe C, Hermans K
Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, and Laboratoire de Microbiologie, Hôpital de Erasme, Brussels, Belgium.
J Vet Med B Infect Dis Vet Public Health. 2006 Nov;53(9):418-22. doi: 10.1111/j.1439-0450.2006.00977.x.
High virulence rabbit Staphylococcus aureus strains cause chronic and spreading problems of mastitis, pododermatitis and subcutaneous abscesses on rabbit flock level, whereas infections with low virulence strains are limited to individual rabbits. In the present report, 13 high virulence rabbit S. aureus strains, selected out of a large collection of strains isolated in five European countries between 1983 and 2004, were genotyped using pulsed-field gel electrophoresis, spa typing, multilocus sequence typing (MLST) and accessory gene regulator (agr) group typing. Two low virulence rabbit S. aureus strains were also included in the study. The results indicate the clonal origin of high virulence rabbit S. aureus strains present in Europe. Furthermore, the results of MLST and spa typing form a basis for international epidemiology of rabbit S. aureus strains, as these DNA sequence-based typing techniques can easily be used for intercentre comparisons.
高毒力兔源金黄色葡萄球菌菌株会在兔群层面引发慢性且扩散性的乳腺炎、足皮炎和皮下脓肿问题，而低毒力菌株感染则局限于个别兔子。在本报告中，从1983年至2004年期间在五个欧洲国家分离出的大量菌株中挑选出13株高毒力兔源金黄色葡萄球菌菌株，采用脉冲场凝胶电泳、spa分型、多位点序列分型（MLST）和辅助基因调节因子（agr）组分型进行基因分型。研究中还纳入了两株低毒力兔源金黄色葡萄球菌菌株。结果表明欧洲存在的高毒力兔源金黄色葡萄球菌菌株具有克隆起源。此外，MLST和spa分型的结果构成了兔源金黄色葡萄球菌菌株国际流行病学研究的基础，因为这些基于DNA序列的分型技术可轻松用于中心间比较。
