Tang Ling, Zhang Ting, Li Xin-zhong
Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410013, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2006 Oct;31(5):756-8.
To establish an high-performance liquid chromatography (HPLC) method for the determination of diltiazem hydrochloride injection.
The assay was conducted on a NUCLEODUR C18 column with buffer solution (pH6.2)-acetonitrile-methanol (40:30:30) as the mobile phase and the detection wavelength was 240 nm.
Diltiazem hydrochloride and relevant substances could be separated under the condition. The linear concentration range of diltiazem hydrochloride was 4 - 200mg/L with the recovery of 100.22% (RSD = 0.37%).
The method may be used for the determination of diltiazem hydrochloride injection.
建立一种测定盐酸地尔硫䓬注射液的高效液相色谱（HPLC）方法。
采用NUCLEODUR C18柱，以缓冲溶液（pH6.2）-乙腈-甲醇（40:30:30）为流动相进行测定，检测波长为240nm。
在此条件下，盐酸地尔硫䓬与有关物质能够分离。盐酸地尔硫䓬的线性浓度范围为4 - 200mg/L，回收率为100.22%（RSD = 0.37%）。
该方法可用于测定盐酸地尔硫䓬注射液。
