Tenuta L M A, Ricomini Filho A P, Del Bel Cury A A, Cury J A
Faculty of Dentistry of Piracicaba, UNICAMP, Piracicaba, SP, Brazil.
Caries Res. 2006;40(6):546-9. doi: 10.1159/000095656.
Microorganisms are selected in dental biofilm by the acidic environment created by sugar fermentation, but the effect of extracellular polysaccharide (EPS) on the counts of cariogenic bacteria is not clear. Dental biofilm was formed in situ for 13 days under exposure 8 times a day to distilled-deionized water, glucose + fructose or sucrose solutions. Mutans streptococci (MS) counts were not different among the groups, but lactobacilli (LB) were significantly higher in glucose + fructose and sucrose groups, without significant difference between them, irrespective of the higher insoluble EPS concentration in the sucrose biofilm matrix. The data suggest that exposure to sugar is more relevant for the predominance of LB in dental biofilm than for MS and that insoluble EPS does not change the counts of these microorganisms in the biofilm.
通过糖发酵产生的酸性环境可在牙菌斑中筛选出微生物，但胞外多糖（EPS）对致龋菌数量的影响尚不清楚。每天8次暴露于蒸馏水、葡萄糖+果糖或蔗糖溶液中，原位形成牙菌斑13天。各组间变形链球菌（MS）数量无差异，但葡萄糖+果糖组和蔗糖组中的乳酸菌（LB）数量显著更高，两组间无显著差异，尽管蔗糖生物膜基质中的不溶性EPS浓度更高。数据表明，糖暴露对牙菌斑中LB占优势比MS更重要，且不溶性EPS不会改变生物膜中这些微生物的数量。
