Funato Tadao, Takeda Mayu
Department of Laboratory Science, School of Health Science, Faculty of Medicine, Kyoto University, Kyoto 606-8507.
Rinsho Byori. 2006 Sep;54(9):910-7.
The real-time reverse transcription polymerase chain reaction (RT-PCR) method is the most sensitive method for the detection of mRNA and DNA virus. The use of this real-time quantitative PCR(RQ-PCR) has been utilized increasingly to monitor gene expression of many types on clinical diagnosis, and has become standard for the detection and quantification of RNA targets. The employment of the techniques for RQ-PCR offers the advantages of high sensitivity and reproducibility. A number of RQ-PCR have been described that commercial PCR kits are available for quantitative analysis of a limited number of clinically important virus only. Quality assurance is necessary to be assessment the overall process and procedures of quantitative PCR as well as the other clinical testing. Therefore, it should be examined to be used with based on appropriately quality control.
实时逆转录聚合酶链反应（RT-PCR）方法是检测mRNA和DNA病毒最灵敏的方法。这种实时定量PCR（RQ-PCR）越来越多地用于监测临床诊断中多种类型的基因表达，并已成为检测和定量RNA靶标的标准方法。RQ-PCR技术的应用具有高灵敏度和可重复性的优点。已经描述了许多RQ-PCR方法，但商业PCR试剂盒仅可用于对少数临床重要病毒进行定量分析。质量保证对于评估定量PCR的整个过程和程序以及其他临床检测是必要的。因此，应基于适当的质量控制来检查其使用情况。
