Bourdineaud J P, Fierobe H P, Lazdunski C, Pagès J M
Centre de Biochimie et de Biologie Moléculaire, CNRS, Marseille, France.
Mol Microbiol. 1990 Oct;4(10):1737-43. doi: 10.1111/j.1365-2958.1990.tb00551.x.
[125I]-colicin N binds to OmpF receptor sites (70,000 per cell) with an average Kassoc of 3.2 x 10(6) M-1 at 23 degrees C. Monoclonal antibody directed against a cell-surface-exposed epitope of OmpF is able to complete with the binding of the colicin in vitro and also to protect against colicin N in vivo. OmpF is an absolute requirement for colicin N uptake. OmpC cannot serve as a substitute for OmpF during translocation across the outer membrane under receptor bypass conditions, which is in contrast to colicin A. Colicin N does not cross-react with various monoclonal antibodies directed against colicin A.
