Silvestris Franco, Cafforio Paola, Calvani Nicola, De Matteo Monica, Lombardi Lucia, Dammacco Franco
Department of Internal Medicine and Oncology (DIMO), University of Bari, Piazza Giulio Cesare 11, 70124 Bari, Italy.
Leuk Lymphoma. 2006 Sep;47(9):1921-31. doi: 10.1080/10428190600649521.
Seven plasma cell lines from patients with smoldering (group A) and overt myeloma (group B) were investigated for both phenotypic markers and in-vitro properties, including sensitivity to apoptosis, cytotoxicity, cell adhesion, chemotaxis and bone interaction. Cell lines from group A underwent apoptosis whereas those from group B were apparently resistant, promoted cytotoxicity in target cells and enhanced both adhesion and migratory functions upon appropriate activators. In addition, MCC-2, a group B cell line from a patient with severe osteolytic disease of the skeleton produced erosive lacunae on bone substrates, whereas this effect was almost absent with cell lines from group A. Concurrent deregulation of relative markers, in combination with peculiar properties including resistance to apoptosis and high cytotoxic potential, as well as adhesion, chemotaxis and bone pathophysiology interactions, may thus identify myeloma cells with aggressive phenotype driving these biological activities in vitro and perhaps in vivo.
对来自冒烟型（A组）和显性骨髓瘤（B组）患者的7种浆细胞系进行了表型标记物和体外特性研究，包括对凋亡的敏感性、细胞毒性、细胞黏附、趋化性和骨相互作用。A组的细胞系发生凋亡，而B组的细胞系明显具有抗性，能促进靶细胞的细胞毒性，并在适当激活剂作用下增强黏附和迁移功能。此外，来自一名患有严重骨骼溶骨性疾病患者的B组细胞系MCC-2在骨基质上产生侵蚀性腔隙，而A组细胞系几乎没有这种作用。因此，相关标记物的同时失调，结合包括对凋亡的抗性和高细胞毒性潜能以及黏附、趋化性和骨病理生理学相互作用等特殊特性，可能识别出具有侵袭性表型的骨髓瘤细胞，这些细胞在体外乃至体内驱动着这些生物学活性。
